ZRB1196

Anti-FAM49A Antibody, clone 2D6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CYFIP-related Rac1 interactor A:CYRIA
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• clone: recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 37.31 kDa; observed mol wt ~37 kDa
• species reactivity: human
• species reactivity (predicted by homology): monkey, rat, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: Q9H0Q0
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 2D6 is ZooMAb rabbit recombinant monoclonal antibody that specifically detects CYFIP-related Rac1 interactor A (FAM49A). It targets an epitope within 24 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human CYFIP-related Rac1 interactor A (FAM49A).

Application

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FAM49A in Human Hippocampus Membrane extractTested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected FAM49A in Human tonsil tissue lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FAM49A in U2OS cells.Flow Cytometry Analysis: 1 μg from a representative lot detected FAM49A in one million U2OS cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FAM49A in human tonsil tissue sections.Affinity Binding Assay: A representative lot of this antibody bound FAM49A peptide with a KD of 5.8 x 10-7 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-FAM49A, clone 2D6 ZooMAb, Cat. No. ZRB1196, is a recombinant Rabbit monoclonal antibody that targets FAM49A and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

CYFIP-related Rac1 interactor A (UniProt: Q9H0Q0; also known as Protein CYRIA, FAM49A) is encoded by the CYRIA (also known as FAM49A) gene (Gene ID: 81553) in human. FAM49A is a evolutionarily conserved protein that is suggested to negatively regulate RAC1 signaling and RAC1-driven cytoskeletal remodeling. It may also regulate chemotaxis, cell migration, and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. It binds to activated Rac1 via a domain of unknown function (DUF1394). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Whitelaw, JA., et al. (2019). Commun. Integr. Biol. 12(1); 112-118).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable