ZRB1034
Anti-ULBP-4/RAET1E Antibody, clone 6C10 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Lymphocyte effector toxicity activation ligand, N2DL-4, NKG2D ligand 4, NKG2DL4, RAE-1-like transcript 4, Retinoic acid early transcript 1E, UL16-binding protein 4
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
6C10, recombinant monoclonal
monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 30.12 kDa
observed mol wt ~26 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... RAET1E(135250)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 6C10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Retinoic acid early transcript 1E. It targets an epitope within the extracellular domain.
Immunogen
His-tagged recombinant fragment corresponding to 195 amino acids from the extracellular domain of human Retinoic acid early transcript 1E (RAET1E).
Application
Anti-ULBP-4/RAET1E, clone 6C10 ZooMAb, Cat. No. ZRB1034, is a recombinant Rabbit monoclonal antibody that specifically targets Retinoic acid early transcript 1E and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ULBP-4/RAET1E in ULBP-4 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ULBP-4/RAET1E in HT-29 cells.
Affinity Binding Assay: A representative lot of this antibody bound human recombinant ULBP-4 protein with a KD of 4.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ULBP-4/RAET1E in HT-29 cells.
Affinity Binding Assay: A representative lot of this antibody bound human recombinant ULBP-4 protein with a KD of 4.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Retinoic acid early transcript 1E (UniProt: Q8TD07; also known as Lymphocyte effector toxicity activation ligand, NKG2D ligand 4, N2DL-4, NKG2DL4, RAE-1-like transcript 4, UL16-binding protein 4, ULBP-4) is encoded by the RAET1E (also known as LETAL, N2DL4, ULBP4, UNQ1867/PRO4303) gene (Gene ID: 135250) in human. ULBP-4 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 31-225), a transmembrane domain (aa 226-248), and a cytoplasmic domain (aa 249-263). It is predominantly expressed in the skin, but also expressed in testis and trachea. Its levels are upregulated in tumor cells of different origins and its expression progressively decreased after treatment of tumor cells with retinoic acid. ULBP-4 also serves as a functional ligand for NKG2D. ULBP-4 is reported to bind and co-stimulate NKG2D-expressing effector cells including NK cells, NKT cells, CD8+ T cells. ULBP-4 has a MHC class I alpha-1 like (aa 31-116) and MHC class I alpha-2 like (117-207) regions that down-regulate the cell surface expression of Killer Cell Lectin Like Receptor K1 (KLRK1). Six different isoforms of ULBP-4 have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chalupny, NJ., (2003). Biochem. Biophys. Res. Commun. 305(1); 129-135).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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