A. Spirin and J. Swartz, John Wiley and sons, 2008, 262 pp., hard cover
manufacturer/tradename
Wiley
General description
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
Table of Contents
Large scale batch reactions for cell-free protein synthesis The constructive approach for cell-free translation Functional genomic analysis using sequential cell-free protein synthesis Cell-free technology for rapid production of patient-specific fusion protein vaccines Bacterial cell-free system for highly efficient protein synthesis The use of the Escherichia-coli cell-free protein synthesis for structural biology and structural proteomics The wheat germ cell-free protein synthesis system Cell-free expression of integral membrane proteins for structural studies Cell-free production of membrane protein in presence of detergents Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering Gene Cloning and Expression in Molecular Colonies
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