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Z731315

Sigma-Aldrich

Cell-free Protein Synthesis

practical advice for beginners and experts alike

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About This Item

ISBN-13:
978-3-527-31649-6
UNSPSC Code:
55101509

publication info

A. Spirin and J. Swartz, John Wiley and sons, 2008, 262 pp., hard cover

manufacturer/tradename

Wiley

General description

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

Table of Contents

Large scale batch reactions for cell-free protein synthesis
The constructive approach for cell-free translation
Functional genomic analysis using sequential cell-free protein synthesis
Cell-free technology for rapid production of patient-specific fusion protein vaccines
Bacterial cell-free system for highly efficient protein synthesis
The use of the Escherichia-coli cell-free protein synthesis for structural biology and structural proteomics
The wheat germ cell-free protein synthesis system
Cell-free expression of integral membrane proteins for structural studies
Cell-free production of membrane protein in presence of detergents
Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering
Gene Cloning and Expression in Molecular Colonies

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