Z730858
Expression Systems
M. Dyson and Y. Durocher, Scion Publishing, 2007, 288 pp., soft cover
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About This Item
ISBN-13:
9781904842439
UNSPSC Code:
55101509
publication info
M. Dyson and Y. Durocher, Scion Publishing, 2007, 288 pp., soft cover
manufacturer/tradename
Cold Spring Harbor
General description
Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when. This book covers expression across a broad range of systems, including the following. *Baculovirus expression vectors, *CHO cells, * E. coli, *HEK293-EBNA1 cells, * Lactococcus lactis, * S. cerevisiae, *transfected insect cells, * Pichia pastoris, *mammalian cells using BacMam viruses, *lentiviral vectors, *wheat germ cell-free system. The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.
Table of Contents
1. Expression strategy
2. Protein expression in Escherichia coli
3. Expression engineering of synthetic antibodies using ribosome display
4. Refolding proteins from inclusion bodies
5. Selection of protein variants with improved expression using GFP-derived folding and solubility reporters
6. Protein expression in the wheat germ cell-free system
7. Saccharomyces cerevisiae ; A microbial eukaryotic expression system
8. Expression of proteins in Pichia pastoris
9. Improved baculovirus expression vectors
10. Transient transfection of insect cells for rapid expression screening and protein production
11. Generation of stable CHO cell lines for protein expression
12. Transient expression in HEK293-EBNA1 cells
13. Nisin- and subtilin-controlled gene expression systems for Gram-positive bacteria
14. Protein expression using lentiviral vectors
15. Expression in mammalian cells using BacMam viruses
List of suppliers
Index
2. Protein expression in Escherichia coli
3. Expression engineering of synthetic antibodies using ribosome display
4. Refolding proteins from inclusion bodies
5. Selection of protein variants with improved expression using GFP-derived folding and solubility reporters
6. Protein expression in the wheat germ cell-free system
7. Saccharomyces cerevisiae ; A microbial eukaryotic expression system
8. Expression of proteins in Pichia pastoris
9. Improved baculovirus expression vectors
10. Transient transfection of insect cells for rapid expression screening and protein production
11. Generation of stable CHO cell lines for protein expression
12. Transient expression in HEK293-EBNA1 cells
13. Nisin- and subtilin-controlled gene expression systems for Gram-positive bacteria
14. Protein expression using lentiviral vectors
15. Expression in mammalian cells using BacMam viruses
List of suppliers
Index
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