Z719218
Whole Genome Amplification: Methods Express Series
Softcover
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About This Item
ISBN-10:
1-90484207-0
ISBN-13:
978-1-90484207-1
UNSPSC Code:
55101509
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publication info
S. Hughes and R. Lasken, Scion Publishing, 2005, 300 pp., soft cover
manufacturer/tradename
Cold Spring Harbor
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General description
Using Whole Genome Amplification (WGA) methods, it is possible to create microgram quantities of DNA starting with as little as one nanogram of genomic DNA and in some cases even a single eukaryotic or bacterial cell. The implementation of such WGA methods provides an ample supply of DNA for large-scale genetic studies. This title provides a comprehensive overview of the field and will be welcomed by all researchers looking to take advantage of the latest developments.
Table of Contents
1.Introduction,
2.Single nucleotide polymorphism typing using degenerated oligonucleotide primer-PCR amplified products as templates
3.WGA by improved primer extension preamplification (I-PEP)
4.Global Amplification using ′Scomp′
5.PRSG, a WGA method based on adaptor-ligation PCR of randomly sheared genomic DNA,
6.Omniplex WGA,
7.DNA Linear Amplification,
8.Multiple-displacement amplification (MDA) from clinical samples or extracted DNA,
9.MDA from single bacterial cells,
10.Genome amplification tolerant to sample degredation: application to FFPE specimens,
11.Preimplantation genetic diagnosis using WGA,
2.Single nucleotide polymorphism typing using degenerated oligonucleotide primer-PCR amplified products as templates
3.WGA by improved primer extension preamplification (I-PEP)
4.Global Amplification using ′Scomp′
5.PRSG, a WGA method based on adaptor-ligation PCR of randomly sheared genomic DNA,
6.Omniplex WGA,
7.DNA Linear Amplification,
8.Multiple-displacement amplification (MDA) from clinical samples or extracted DNA,
9.MDA from single bacterial cells,
10.Genome amplification tolerant to sample degredation: application to FFPE specimens,
11.Preimplantation genetic diagnosis using WGA,
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