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WH0246243M1

Sigma-Aldrich

Monoclonal Anti-RNASEH1 antibody produced in mouse

clone 5D10, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-H1RNA, Anti-ribonuclease H1

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100 μG
$465.26

$465.26

List Price$541.00Save 14%
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100 μG
$465.26

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

$465.26

List Price$541.00Save 14%
Ships within 2 weeks. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5D10, monoclonal

form

buffered aqueous solution

species reactivity

human, rat, mouse

technique(s)

indirect ELISA: suitable
western blot: 1-5 μg/mL

isotype

IgG2aκ

GenBank accession no.

NM_002936

UniProt accession no.

O60930

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RNASEH1(246243)

General description

Ribonuclease H1 (RNASEH1) is an endonuclease which is expressed in the mitochondria and nucleus. It possesses two domains which aid in binding to nucleic acids. The gene encoding this protein is localized on human chromosome 2p25.

Immunogen

RNASEH1 (NP_002927, 189 a.a. ~ 286 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
AACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Biochem/physiol Actions

Ribonuclease H1 (RNASEH1) digests the RNA segment in a DNA-RNA hybrid. Mutations in the gene encoding it have been associated with chronic progressive external ophthalmoplegia.

Physical form

Solution in phosphate buffered saline, pH 7.4

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
06335-5D10

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Aurelio Reyes et al.
American journal of human genetics, 97(1), 186-193 (2015-06-23)
Chronic progressive external ophthalmoplegia (CPEO) is common in mitochondrial disorders and is frequently associated with multiple mtDNA deletions. The onset is typically in adulthood, and affected subjects can also present with general muscle weakness. The underlying genetic defects comprise autosomal-dominant
E J Devor et al.
The Journal of heredity, 95(3), 257-261 (2004-06-29)
A retroprocessed pseudogene (retropseudogene) descended from the gene encoding ribonuclease (RNase) H1 has been found in ape genomes that preserves a splice junction mutation event that altered the carboxyl-terminal end of the enzyme. The GT --> GC transition mutant at
Christopher N Johnson et al.
Biochemistry, 50(19), 3903-3912 (2011-03-30)
Numerous DNA chemistries for improving oligodeoxynucleotide (ODN)-based RNA targeting have been explored. The majority of the modifications render the ODN/RNA target insensitive to RNase H1. Borano phosphonate ODN's are among the few modifications that are tolerated by RNase H1. To
So Hae Park et al.
Nucleic acids research, 49(13), 7507-7524 (2021-06-29)
Impaired replication progression leads to de novo copy number variant (CNV) formation at common fragile sites (CFSs). We previously showed that these hotspots for genome instability reside in late-replicating domains associated with large transcribed genes and provided indirect evidence that

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