A component of mesenchymal cell intermediate filaments.
Reconstitution
When reconstituted with distilled water at 1 mg vimentin per mL, the solution will contain 10 mM sodium phosphate, pH 7.5, 2 mM dithiothreitol, 1 mM EDTA, 6 M urea, and 10 mM methylammonium chloride.
A novel, simple and relatively rapid method is described for the isolation of the intermediate-sized filament protein vimentin from eye lens tissue. Chromatofocusing is applied as the sole purification step. The apparent isoelectric point of the protein in 6 M
Epithelial ovarian cancer is an aggressive and deadly disease and understanding its invasion mechanisms is critical for its treatment. We sought to study the penetration/invasion of ovarian tumor cells into extracellular matrices (ECMs) using a fibroblast-derived three-dimensional (3D) culture model
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