V1010
Anti-Vascular Endothelial Growth Factor B 167/186 antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-VEGF-B 167/186
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About This Item
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
technique(s)
indirect ELISA: 0.5-1.0 μg/mL
western blot: 0.1-0.2 μg/mL
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VEGFB(7423)
General description
The vascular endothelial growth factor family consists of five members, VEGF-A to VEGF-E. VEGF-B has a structure that is similar to VEGF-A and the placenta growth factor. It is highly expressed in most tissues and organs and most abundantly in heart. VEGF-B binds and signals via the VEGFR-1 receptor and neuropilin-1 coreceptor. Even though it belongs to the family of growth factors that induce angiogenesis and are necessary for formation of vasculature, VEGF-B differs in acting as a survival factor and less of angiogenic factor. The survival effect is pleotropic and also affects neurons, vascular cells and cardiac myocytes. There are two isoforms of VEGF-B that result from alternate splicing, VEGF-B 167 and VEGF-B 186. VEGF-B 167 binds heparin and is associated with extracellular matrix whereas VEGF-B 186 is a secreted form. Anti-Human Vascular Endothelial Growth Factor B recognizes human VEGF-B 167 and human VEGF-B 186. The antibody shows approximately 30% cross reactivity with recombinant mouse VEGF-B 165 and VEGF-B 186. It exhibits less than 5% cross reactivity with recombinant human VEGF-D and no cross reactivity with recombinant human VEGF and recombinant mouse VEGF.
Immunogen
recombinant human vascular endothelial growth factor B 186 expressed in E. coli. Purified by passing goat sera over a human VEGF-B 186 affinity column and then passing the bound fraction over a human VEGF-B 167 affinity column.
Application
Anti-Vascular Endothelial Growth Factor B 167/186 antibody may be used for immunoblotting at a working antibody concentration of 0.1-0.2 μg/ml. For ELISA, a concentration of 0.5-1.0 μg/ml is recommended.
Physical form
Lyophilized from a 2 μm filtered solution in phosphate buffered saline.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Xuri Li et al.
Cell adhesion & migration, 3(4), 322-327 (2009-08-18)
Despite its early discovery and high sequence homology to the other VEGF family members, the biological function of VEGF-B remained debatable for a long time, and VEGF-B has received little attention from the field thus far. Recently, we and others
B Olofsson et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(6), 2576-2581 (1996-03-19)
We have isolated and characterized a novel growth factor for endothelial cells, vascular endothelial growth factor B (VEGF-B), with structural similarities to vascular endothelial growth factor (VEGF) and placenta growth factor. VEGF-B was particularly abundant in heart and skeletal muscle
B Olofsson et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(20), 11709-11714 (1998-09-30)
The vascular endothelial growth factor (VEGF) family has recently expanded by the identification and cloning of three additional members, namely VEGF-B, VEGF-C, and VEGF-D. In this study we demonstrate that VEGF-B binds selectively to VEGF receptor-1/Flt-1. This binding can be
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