U6875

Uridine 5′-triphosphate tris salt

Type VI, ≥90%

• Synonym(s): UTP
• Empirical Formula (Hill Notation): C₉H₁₅N₂O₁₅P₃
• CAS Number: 108321-53-5
• Molecular Weight: 484.14
• MDL number: MFCD00058360
• UNSPSC Code: 12352200
• PubChem Substance ID: 329827645
• NACRES: NA.77

Properties

• type: Type VI
• assay: ≥90%
• solubility: H₂O: soluble 50 mg/mL, clear, colorless
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: NC(CO)(CO)CO.O[C@H]1[C@@H](O)[C@@H](O[C@@H]1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=CC(=O)NC2=O
• InChI: 1S/C9H15N2O15P3.C4H11NO3/c12-5-1-2-11(9(15)10-5)8-7(14)6(13)4(24-8)3-23-28(19,20)26-29(21,22)25-27(16,17)18;5-4(1-6,2-7)3-8/h1-2,4,6-8,13-14H,3H2,(H,19,20)(H,21,22)(H,10,12,15)(H2,16,17,18);6-8H,1-3,5H2/t4-,6-,7-,8-;/m1./s1
• InChI key: NHMNWWVJZZPKSL-IAIGYFSYSA-N

Description

Application

UTP has been used to study intracellular Ca²⁺ concentration in alveolar type II (AT2) cells. UTP has also been used as a phosphate donor for in vitro kinase assays using deoxycytidine kinase (DCK) or whole cell lysates.

Uridine 5′-triphosphate tris salt has been used:

• to investigate its activation potential on the purinergic P2Y2 Receptor in THP1 monocytes
• as a component of non-radioactive nucleoside triphosphate solution used in in vitro RNA polymerase assay
• as a P2Y2 agonist in human embryonic kidney cells (HEK293T) in dynamic mass redistribution (DMR) assays

Biochem/physiol Actions

Uridine 5′-triphosphate (UTP) is synthesized from uridine 5′-diphosphate (UDP) in the presence of the enzyme nucleoside diphosphate kinase (NDPK). Plant cells have high uridine as UTP. UTP on amination results in the generation of CTP and is catalysed by enzyme CTP synthetase. UTP acts on the purinergic (P2Y2) receptor and mediates smooth muscle cells (SMCs) mitogenic activation. It also modulates osteopontin expression in SMCs. UTP is a sputum inducer and a promising candidate for assessing the inflammation in respiratory airway.

P2Y receptor agonist.

Preparation Note

Uridine 5′-triphosphate (UTP) tris salt is soluble in water at 50 mg/ml and yields a clear, colorless solution.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges