TSTOPRT

ThermaStop^™-RT

increases RT-PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before RT-PCR

• Synonym(s): Multiplex RT-PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart
• UNSPSC Code: 12352200

Properties

• form: lyophilized powder
• packaging: pkg of 2500 units; pkg of 500 units
• technique(s): RT-PCR: suitable
• color: opaque
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

ThermaStop^™-RT is a proprietary additive for One-Step, or Two-Step Reverse Transcriptase-PCR assays containing gene-specific primers. ThermaStop^™-RT interacts with the reverse transcriptase at low temperatures to reduce priming errors. ThermaStop^™-RT improves detection sensitivity and specific product yield by eliminating priming errors at low temperature. ThermaStop-RT acts as a Hot-start which binds and inhibits DNA polymerase activity. It also renatures the DNA polymerase upon cooling and activates its functions at lower temperatures or post-amplification.

Application

ThermaStop^™-RT is suitable for gene expression quantitative PCR (qPCR) and next-generation sequencing (NGS) applications.

Features and Benefits

• It eliminates the non-specific product formation
• Provides increased sensitivity and specificity
• Provides higher yield and pure cDNA synthesis for conventional RT-PCR
• It minimizes the primer-dimer formation
• It utilizes the same cycling conditions as conventional Taq polymerase
• Decreases the RNA secondary structure
• Stable at low temperatures
• Enzymes are inhibited at below 55°C
• Compatible with several RT-PCR enzymes
• Provides better multiplexing, efficient primer use
• Better quantitative accuracy and monoplex and multiplex amplification sensitivity

Packaging

One unit of ThermaStop^™-RT is defined as the amount required for optimal performance in a one-step RT-PCR containing 50 units of reverse transcriptase (RT) and one unit of hot-start Taq Polymerase in a volume of 20 uL and a temperature of 50 degrees C.

Other Notes

RT-PCR, *Please note ThermaStop^™-RT should not be used in combination with ThermaStop^™ or ThermaGo^™.

Reconstitution

Dissolve in Tris pH 8.3 buffer

Legal Information

ThermaGo is a trademark of ThermaGenix Inc.

ThermaStop is a trademark of ThermaGenix Inc.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable