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TSTOP

Sigma-Aldrich

ThermaStop™

increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before PCR, improves hot start PCR

Synonym(s):

Multiplex PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart

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About This Item

UNSPSC Code:
12352200

form

lyophilized powder

packaging

pkg of 2500 units
pkg of 500 units
pkg of One unit of ThermaStop™ is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 μl.

technique(s)

PCR: suitable

color

opaque

shipped in

ambient

storage temp.

2-8°C

General description

ThermaStop™ is a reversible hot-start reagent that is compatible with thermostable DNA polymerases. It acts directly on the polymerase to prevent non-specific enzymatic activity below 50°C. Polymerase activity is fully restored at 60°C, but is inhibited again by ThermaStop™ by cooling the reaction. Adding ThermaStop™ to your PCR reactions improves sensitivity and total product yield.

Application

ThermaStop™ has been used as a component of the PCR mix for the human immunodeficiency virus (HIV-1) proviral genome amplification by non-multiplexed nested PCR.[1] It has also been used for the amplification of DNA from oral swabs of HIV infected individuals.[2]

Features and Benefits

  • Adding ThermaStop™ to your PCR reactions improves sensitivity and total product yield
  • Increases the product yield for downstream applications like genotyping, next-generation sequencing (NGS) sample preparation, Gibson assembly, diagnostics, and multiplex PCR
  • It improves polymerase specificity, enhances PCR performance and quantitative data
  • Prevents primer-dimer formation and non-specific products before PCR
  • Enables stabilization of master mixes at room temperature before PCR for up to 36 hrs
  • Rapidly inactivated during the first PCR cycle
  • Rapidly re-activated upon cooling

Reconstitution

Dissolve in Tris pH 8.3 buffer

Legal Information

ThermaStop is a trademark of ThermaGenix Inc.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Jan Clement Santiago et al.
PLoS pathogens, 17(1), e1008594-e1008594 (2021-01-20)
Intra-host tumor virus variants may influence the pathogenesis and treatment responses of some virally-associated cancers. However, the intra-host variability of Kaposi sarcoma-associated herpesvirus (KSHV), the etiologic agent of Kaposi sarcoma (KS), has to date been explored with sequencing technologies that
Basiel Cole et al.
Nature communications, 12(1), 3727-3727 (2021-06-19)
Clonal expansion of HIV-infected cells contributes to the long-term persistence of the HIV reservoir in ART-suppressed individuals. However, the contribution from cell clones that harbor inducible proviruses to plasma viremia is poorly understood. Here, we describe a single-cell approach to

Articles

ThermaStop™ PCR Additive

PCR additive added to master mix that stops nonspecific bands after cool down.

ThermaGenix Product Overview

How to stop nonspecific bands and off target primers in PCR.

ThermaStop™-RT PCR Additive

PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.

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