PVA polymers are functionalized with thiol-reactive maleimide groups, which react rapidly with thiol groups of crosslinkers to form hydrogels in few seconds. The thiol-reactive maleimide groups are also compatible to conjugate polymers with bioactive factors. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels. The buffers (pH7.2 and pH 5.5) are used to control pH and osmotic conditions to optimize time for gelation.
Application
FAST-PVA polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels.
Features and Benefits
Contains synthetic polymer (PVA) functionalized with maleimide groups for quick gelation
Non degradable polymer
Components
TRUEPVAF contains FAST-PVA solution in phosphate buffer, TrueGel3D thioglycerol and water.
Caution
Avoid frequent freeze-thaw cycles and store FAST-PVA polymer in small portions.
TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions
An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation
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