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TRUECD

Sigma-Aldrich

TrueGel3D Crosslinker

CD cell-degradable crosslinker

Synonym(s):

Crosslinker

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.52

shipped in

dry ice

storage temp.

−20°C

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General description

CD cell-degradable crosslinker is comprised of conjugated peptide along with polyethylene glycol and thiol (SH) groups. The thiol groups form stable thioether bond with thiol-reactive groups of the polymers (Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran) and results in a stable hydrogel.

The conjugated peptide has matrix metalloprotease (MMP)-cleavage sites (Pro-Leu-Gly-Leu-Trp-Ala), which allows matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9) to locally degrade the polymer network. It allows cells to spread and migrate within the gel if they express the indicated MMPs.

Application

TRUECD is used to crosslink PVA and dextran polymers to generate cell-degradable hydrogel

Features and Benefits

MMP degradable

Components

TRUECD contains CD cell-degradable crosslinker lyophilized and water.

Caution

Avoid prolong exposure of CD cell-degradable crosslinker to air

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Protocols

TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.

TrueGel3D hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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