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TRUE2

Sigma-Aldrich

TrueGel3D Hydrogel Kits

FAST-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

shipped in

dry ice

storage temp.

−70°C

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General description

The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on PEG non cell-degradable crosslinker. Maleimide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD (TRUERGD-1UM) prior to the crosslinking step.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

TRUE2-1KT is used to grow cells that don′t attach to hydrogel for survival but rather use their secreted extracellular matrix. It is used to immobilize cells or specimens for imaging and for bioprinting.

Features and Benefits

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Allow cell recovery and post-culture analyses
  • Flexibility in modulating gel stiffness
  • Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)
  • Does not allow cell migration

Components

TRUE2-1KT contains lyophilized FAST-DEXTRAN (polymer) and PEG non cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol, and water.

Caution

Avoid prolong exposure of PEG non cell-degradable crosslinker to air.

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

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Articles

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

Protocols

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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