T9700

Monoclonal Anti-TWEAK Receptor/Fn-14 antibody produced in mouse

~2 mg/mL, clone TR-22, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-TNF-like Weak Inducer of Apoptosis Receptor
• MDL number: MFCD07785416
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TR-22, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~14 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): flow cytometry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 2-4 μg/mL using HEK-293 overexpressing TWEAKR cells lysate
• isotype: IgG1
• UniProt accession no.: Q9NP84
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TNFRSF12A(51330)

Description

General description

Monoclonal Anti-TWEAK Receptor (mouse IgG1 isotype) is derived from the TR-22 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with human TWEAK receptor. Tumor necrosis factor receptor superfamily member 12A or TNF-like weak inducer of apoptosis Receptor (TWEAKR) or fibroblast growth factor-inducible immediate-early response protein 14 (fn14) is a type I transmembrane receptor comprising transmembrane domain, amino-terminal signal peptide, and carboxy-terminal cytoplasmic domain. The TWEAKR gene is mapped to human chromosome 16p13.3. TWEAKR is expressed in the placenta, lungs, kidney, and heart.

Specificity

Monoclonal Anti-TWEAK receptor recognizes human TWEAK receptor

Immunogen

synthetic peptide corresponding to amino acids 39-52 in the N-terminus of human TWEAK receptor.

Application

Monoclonal Anti-TWEAK Receptor/Fn-14 antibody produced in mouse is suitable for:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• flow cytometry

Biochem/physiol Actions

Fn14 induces growth effects in neurons independent of the ligand, TWEAK.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recom-mended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)