By immunoblotting against the reduced protein, the antibody reacts with bands at approximately 21 kDa. It also reacts with non-reduced TIMP-2.
Immunogen
synthetic peptide corresponding to the first loop of human tissue inhibitor of metalloproteinase-2
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 50% glycerol and 15 mM sodium azide.
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Laboratory investigation; a journal of technical methods and pathology, 82(11), 1583-1590 (2002-11-14)
Gelatin zymography is the common method for examining matrix metalloproteinase-2 (MMP-2) in cells and media samples. Activation of the latent MMP-2 zymogen involves its binding to the cell surface MT1-MMP*TIMP-2 (membrane type-1 matrix metalloproteinase/tissue inhibitor of matrix metalloproteinase-2) complex with
Biology of reproduction, 66(5), 1219-1229 (2002-04-23)
The testicular and epididymal fluids of ram, boar, and stallion were analyzed by means of one-dimensional and two-dimensional gelatin gel zymography. Five main gelatinolytic bands were revealed in the ram and at least seven were observed in the boar and
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