T5817
Monoclonal Anti-TCF-4 (T-Cell Factor-4) antibody produced in mouse
~1 mg/mL, clone 6H5-3, purified immunoglobulin, buffered aqueous glycerol solution
Synonym(s):
Anti-TCF4
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6H5-3, monoclonal
form
buffered aqueous glycerol solution
mol wt
antigen 66 kDa
species reactivity
human, mouse
concentration
~1 mg/mL
technique(s)
immunohistochemistry: suitable using 4% formaldehyde, paraffin-embedded human and mouse tissue
immunoprecipitation (IP): suitable
western blot: 1-4 μg/mL using whole extracts of Cos-1 cells transfected with human TCF-4
isotype
IgG2a
UniProt accession no.
shipped in
wet ice
Storage temp.
−20°C
Gene Information
human ... TCF7L2(6934)
mouse ... Tcf7l2(21416)
General description
Transcription factor 4 or T-Cell Factor-4 (TCF-4), also called as transcription factor 7 like 2 (TCF7L2), is mapped to human chromosome 18q21.1. The gene codes for a basic helix-loop-helix DNA binding protein, which belongs to TCF family and is implicated in transcriptional regulation. It is expressed predominantly in nervous system. TCF4 protein has a transcriptional activation domain 2 (AD2), a transcriptional activation domain (AD1) and a nuclear localization signal (NLS) in the N-terminal end and a C-terminal basic helix-loop-helix (bHLH) domain, involved in dimerization and deoxyribonucleic acid (DNA) binding.
Immunogen
His-tagged fusion protein corresponding to amino acids 31-331 of human TCF-4
Application
May be used for gel-shift assays
Biochem/physiol Actions
Transcription factor 4 (TCF-4) interacts functionally with β-catenin to mediate Wnt signaling pathway and transactivates downstream target genes implicated in cancer development. TCF-4 plays a vital role in development of the nervous system and vertebrate development. Aberrations or mutation of the gene leads to Pitt-Hopkins syndrome (PTHS), a rare developmental disorder characterized by severe motor and mental retardation, typical facial features and breathing abnormalities.
Physical form
Solution in 0.7 M tris-glycine, pH 7.4, containing 0.105 M sodium chloride, 30% glycerol and 0.035% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Chun-Fu Hong et al.
The Journal of biological chemistry, 284(29), 19613-19622 (2009-05-16)
T cell factor 4 (TCF4) interacts with beta-catenin in the WNT signaling pathway and transactivates downstream target genes involved in cancer progression. To identify proteins that regulate TCF4-mediated biological responses, we performed a yeast two-hybrid screen to search for a
Lili Zhou et al.
The American journal of pathology, 185(12), 3274-3289 (2015-10-21)
γ-Catenin, an important component of desmosomes, may also participate in Wnt signaling. Herein, we dissect the role of γ-catenin in liver by generating conditional γ-catenin knockout (KO) mice and assessing their phenotype after bile duct ligation (BDL) and diethylnitrosamine-induced chemical
Mari Sepp et al.
Human molecular genetics, 21(13), 2873-2888 (2012-03-31)
Transcription factor TCF4 (alias ITF2, SEF2 or E2-2) is a broadly expressed basic helix-loop-helix (bHLH) protein that functions as a homo- or heterodimer. Missense, nonsense, frame-shift and splice-site mutations as well as translocations and large deletions encompassing TCF4 gene cause
Kayoko Takeda et al.
BMC cancer, 8, 328-328 (2008-11-11)
A recent study has shown that phosphorylated c-Jun (p-c-Jun) interacts with TCF4 to form a complex that cooperatively enhances their transcriptional activity in the presence of beta-Catenin, and that their interaction is critical for mouse intestinal tumorigenesis. To determine the
Mohamed El-Tanani et al.
The Journal of biological chemistry, 279(20), 20794-20806 (2004-03-03)
Osteopontin (OPN) is a multifunctional protein implicated in mammary development, neoplastic change, and metastasis. OPN is a target gene for beta-catenin-T cell factor signaling, which is commonly disturbed during mammary oncogenesis, but the understanding of OPN regulation is incomplete. Data
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