T2827

Anti-dTAF80 antibody, Mouse monoclonal

clone 3D10, purified from hybridoma cell culture

• Synonym(s): Anti-TAF5, Anti-TAF80/85, Anti-TAFII80, Anti-dmTAF5
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 3D10, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: Drosophila melanogaster
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): indirect ELISA: suitable; western blot: 8-16 μg/mL using nuclear extracts of D.Mel cells.
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: Drosophila melanogaster ... Taf5(47900)

Description

General description

Monoclonal Anti-dTAF80 (mouse IgG2b isotype) is derived from the hybridoma 3D10 produced by the fusion of mouse myeloma cells and splenocytes from mice. Drosophila tightly associated factor 80 (dTAF80), also known as tightly associated factor 5 (TAF5) consists of numerous copies ofβ−transducin (WD40) repeat.

Specificity

Monoclonal Anti-dTAF80 reacts specifically with Drosophila dTAF80

Immunogen

Drosophila TFIID complex.

Application

Anti-dTAF80 antibody, mouse monoclonal may be used in immunoblotting and enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Drosophila tightly associated factor 80 (dTAF80) recognizes and interacts with a higher-order complex containing TATA box-binding protein (TBP), delta N250, 110 and 60.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples shouldbe discard if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter