T2450
Anti-TrkC antibody produced in goat
affinity isolated antibody
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About This Item
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
flow cytometry: 3-10 μg/mL
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15 μg/mL
western blot: 0.1-0.2 μg/mL
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... NTRK3(4916)
General description
The development and maintenance of central and peripheral nervous system is controlled by a family of Trk family of neurotrophin receptors. Neurotrophins have an important role in development of the nervous system and mediate the cellular effects by binding to the Trk receptors. The Trk family consists of 3 members, TrkA (NTRK1), Trk B (NTRK2) and TrkC (NTRK3). Neurotrophin-3 (NT3) is the primary ligand for TrkC receptor, but it can also bind and activate the other Trk members.Trk receptors reportedly play a major role in the development and behaviour of neuroblastomas and other type of cancers. TrkC is expressed in at least 25% of primary neuroblastomas. Trk members have been observed to be over-expressed in congenital fibrosarcoma, papillary thyroid carcinomas, pediatric carcinomas and leukemias. Aberrations in TrkC gene have been reported in secretory breast cancers and acute myelogenous leukemia
Anti-TrkC recognizes human TrkC. The antibody shows less than 1% cross-reactivity with human TrkB.
Anti-TrkC recognizes human TrkC. The antibody shows less than 1% cross-reactivity with human TrkB.
Immunogen
purified recombinant human TrkC extracellular domain, expressed in NSO cells.
Application
Anti-TrkC may be used to detect human TrkC by immunoblotting using a concentration of 0.1-0.2 μg/mL. Neutralization of receptor-ligand interaction may be possible at a working concentration of 1-3 μg/mL which blocks 50% of the ligand binding. Neutralization of TrkC has been demonstrated in three-dimensional cultures of rat Sertoli cells. A working concentration of 15 μg/mL may be used for detection of human TrkC in paraffin-embedded sections. For flow cytometry applications, antibody concentration of 3-10 μg/mL/106 is recommended.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Rearrangements of RET and NTRK1 tyrosine kinase receptors in papillary thyroid carcinomas
Pierotti MA et al
Rec. Res. Canc. Res., 154, 237-247 (1998)
D J Yamashiro et al.
Oncogene, 12(1), 37-41 (1996-01-04)
Human neuroblastomas have been found to express the neurotrophin receptors TrkA and TrkB. Expression of TrkA correlates with favorable outcome, while expression of full-length TrkB is associated with unfavorable, more aggressive, N-myc amplified tumors. In this study we have determined
R Klein et al.
Cell, 65(1), 189-197 (1991-04-05)
Two classes of receptors with distinct affinities for nerve growth factor (NGF) have been identified. The low affinity receptor (Kd approximately 10(-9) to 10(-8) M) is a cysteine-rich glycoprotein encoded by the previously characterized LNGFR gene. The structural nature of
R Klein et al.
Nature, 368(6468), 249-251 (1994-03-17)
The trkC gene is expressed throughout the mammalian nervous system and encodes a series of tyrosine protein kinase isoforms that serve as receptors for neurotrophin-3 (NT3), a member of the nerve growth factor (NGF) family of neurotrophic factors. One of
D R Kaplan et al.
Nature, 350(6314), 158-160 (1991-03-14)
Nerve growth factor (NGF) is a neurotrophic factor responsible for the differentiation and survival of sympathetic and sensory neurons as well as selective populations of cholinergic neurons. NGF binds to specific cell-surface receptors but the mechanism for transduction of the
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