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T1788

Sigma-Aldrich

Tyrode′s Solution, Acidic

liquid, sterile-filtered, suitable for mouse embryo cell culture

Synonym(s):

Cell culture solution

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100 ML
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100 ML
$59.40

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

$59.40


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Request a Bulk Order

sterility

sterile-filtered

Quality Level

form

liquid

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

pH

2.5±0.3

shipped in

dry ice

storage temp.

−20°C

General description

Tyrode′s salt solution is used for diluting protein components of media of natural origin. The current role of a balanced salt solution in cell culture is multifaceted. It serves as an irrigating, transporting, and diluting agent while maintaining intra- and extracellular osmotic balance and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It also provides the essential energy for cell metabolism and a buffering system to maintain the medium within the optimal pH range (7.2-7.6).

application

Tyrode′s Solution, Acidic has been used for the removal of zona pellucida from oocytes/embryos.[1][2][3]

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Sara Carvalhal et al.
Molecular biology of the cell, 28(19), 2470-2478 (2017-08-05)
Asymmetric cell divisions depend on the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center, but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small
Zhao-Yang Xu et al.
Scientific reports, 5, 16978-16978 (2015-11-20)
Cep55 is a relatively novel member of the centrosomal protein family. Here, we show that Cep55 is expressed in mouse oocytes from the germinal vesicle (GV) to metaphase II (MII) stages. Immuostaining and confocal microscopy as well as time lapse
Guojing Chang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(7), 2760-2770 (2017-03-16)
During pregnancy, placental trophoblasts at the feto-maternal interface produce a broad repertoire of microRNA (miRNA) species. These species include miRNA from the primate-specific chromosome 19 miRNA cluster (C19MC), which is expressed nearly exclusively in the placenta. Trafficking of these miRNAs
Yao-Long Yan et al.
PLoS biology, 17(6), e3000324-e3000324 (2019-06-22)
The molecular mechanism controlling the zygotic genome activation (ZGA) in mammals remains poorly understood. The 2-cell (2C)-like cells spontaneously emerging from cultures of mouse embryonic stem cells (ESCs) share some key transcriptional and epigenetic programs with 2C-stage embryos. By studying
Tomonori Nakamura et al.
Scientific data, 4, 170067-170067 (2017-06-27)
In mammals, the development of pluripotency and specification of primordial germ cells (PGCs) have been studied predominantly using mice as a model organism. However, divergences among mammalian species for such processes have begun to be recognized. Between humans and mice

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Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

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