T1077

Tissue Inhibitor of Metalloproteinase-2 human

recombinant, expressed in CHO cells, >95% (SDS-PAGE), lyophilized powder

• Synonym(s): TIMP-2
• MDL number: MFCD00285923
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in CHO cells
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: apparent mol wt ~20 kDa
• technique(s): activity assay: suitable; inhibition assay: suitable
• UniProt accession no.: P16035
• storage temp.: −20°C
• Gene Information: human ... TIMP2(7077)

Description

General description

Tissue inhibitor of metalloproteinase-2 (TIMP-2) is an extracellular inhibitor of matrix metalloproteinases (MMPs). There are six conserved cysteine residues forming disulfide loops in its amino- and carboxy-terminal domains. The gene encoding this protein is localized on human chromosome 17q25.[1]

Biochem/physiol Actions

TIMP-2 has a greater binding efficiency to MMP-2 than the other MMPs. Although TIMP-2 is an inhibitor of MMP-2, it is also required at low concentrations for the activation of MMP-2.

Tissue inhibitor of metalloproteinase-2 (TIMP-2), like other protease inhibitors, functions as a key modulator of extracellular matrix degradation during tissue development and remodeling.[2] TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro.[3] It demonstrates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22.[4]

Physical form

Lyophilized from a 0.2 μm filtered solution in 25 mM Tris, and 150 mM sodium chloride, pH 7.5.

Analysis Note

The biological activity is measured by the ability to inhibit human MMP-2 hydrolysis of a fluorogenic MMP substrate.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves