This antibody can be used with both formalin-fixed paraffin-embedded (FFPE) tissue sections and frozen sections.
T0438
Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse
clone 9016, purified immunoglobulin, lyophilized powder
Synonym(s):
Anti-TGF-β1, CEDLAP, DPD1, latency-associated peptide
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9016, monoclonal
form
lyophilized powder
species reactivity
mouse, human, rat
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
application(s)
research pathology
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TGFB1(7040)
mouse ... Tgfb1(21803)
rat ... Tgfb1(59086)
General description
Transforming Growth Factor-β1 (TGFβ1) is mapped to human chromosome 19q13.2.
Transforming growth factor-β (TGF-β) belongs to the TGF-β superfamily of secreted proteins. TGF-β 1 exists as three isoforms TGF-β 1, TGF-β 2 and TGF-β 3. The TGF-β family is involved in a variety of signaling pathways that involve serine/threonine protein kinases. TGF-β acts through binding to three transmembrane receptors in mammals, type I, type II and type III receptors. The important pathways regulated by TGF-β are the SMAD, BMP, MAPK and PI3K/Akt pathways. TGF-β 1 is involved in cell and tissue differentiation, proliferation, inflammation, migration of monocytes, neutrophils and chemotaxis. Deregulation of TGF-β signaling has been implicated in many inflammation and autoimmune diseases. Monoclonal Anti-Transforming Growth Factor-β 1 antibody specifically reacts with human, mouse and rat TGF-β 1. The antibody shows less than 2% cross reactivity with TGF-β3 and TGF-β5 in ELISA.
Specificity
By capture ELISA, <2% cross-reactivity is observed with TGF-β3 and TGF-β5.
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant TGF- beta 1 and latent TGF- beta 1
Application
Recommended concentrations:
Western Blot 1 ug/mL (under non-reducing conditions only)
Immunohistochemistry 3-25 μg/mL
Flow Cytometry 2.5 μg/106 cells
ELISA capture 2-8 μg/mL
ELISA detection 0.1-0.4 μg/mL
Neutrilization 0.3-1.0 μg/mL
Western Blot 1 ug/mL (under non-reducing conditions only)
Immunohistochemistry 3-25 μg/mL
Flow Cytometry 2.5 μg/106 cells
ELISA capture 2-8 μg/mL
ELISA detection 0.1-0.4 μg/mL
Neutrilization 0.3-1.0 μg/mL
Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse has been used in:
- immunohistochemical detection of TGFβ1 in smooth muscle(2) and liver sections
- western blotting and ELISA of brain specimens
- immunoblot detection of TGFβ1 in macrophages
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Other Notes
Protein A or G purified from hybridoma culture supernatant.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used for immunohistochemistry with frozen and/or formalin-fixed paraffin-embedded sections?
1 answer-
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How is Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, different than T0688, Anti-Transforming Growth Factor-?1 antibody produced in chicken?
1 answer-
These two antibodies are very similar in regards to the applications they are suitable for. They do differ in a few key areas. T0688 only recognizes active TGF-ß1 while T0438 recognizes both active and latent TGF-ß1. Also, T0438 is a mouse monoclonal antibody and T0688 is a chicken polyclonal antibody.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What is the molecular weight of Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse,?
1 answer-
TGF-ß1 is a disulfide-linked homodimer of two 12.5 kDa subunits. Under non-reducing conditions, the T0438 antibody should detect a protein at 25 kDa.
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Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used to neutralize the biological activity of TGF-ß1?
1 answer-
Yes, this antibody will neutralize TGF-ß1.
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Will Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, work for intracellular flow cytometry?
1 answer-
This antibody can be used for intracellular flow cytometry. The cells should be fixed with 4% paraformaldehyde and permeabilized with a 0.1% saponin-containing buffer.
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