SRP6531

NMNAT3 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(s): EC 2.7.7.1, EC 2.7.7.18, NMN Adenylyltransferase 3, NaMN Adenylyltransferase 3, Nicotinamide Mononucleotide Adenylyl-transferase 3
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: liquid
• mol wt: monomer ~270 kDa (Human full-length NMNAT3 aa 1-252 is fused at the N-terminus to a His-tag.)
• packaging: pkg of 10 and 50 μg
• concentration: 1 mg/mL
• UniProt accession no.: Q96T66
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... NMNAT3(349565)

Description

General description

This is the Mitochondrial NMNAT isoform. It catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. It can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. It can use tiazofurin monophosphate as substrate. It can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD (+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. It fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. It protects against axonal degeneration following injury.

Physical form

1 mg/mL in 50 mM sodium phosphate, pH 8.0 containing 300 mM sodium chloride, 1 mM DTT and 10% glycerol.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable