SRP6496

CD85J/LILRB1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): CD85, ILT2, LIR1, MIR7
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 73.8 kDa (This protein is fused with Fc fragment of human IgG1 at the C-terminus.); observed mol wt 90-110 kDa (DTT-reduced. Protein migrates due to glycosylation. Gly 24 is the predicted N-terminal.)
• packaging: pkg of 10 and 50 μg; pkg of 50 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: Q8NHL6
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... LILRB1(10859)

Description

General description

CD85 antigen-like family member J (CD85J) also known as Leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1 or LIR-1), Immunoglobulin-like transcript 2 (ILT-2), Monocyte/macrophage immunoglobulin-like receptor 7 (MIR7), which belongs to leukocyte immunoglobulin-like receptor (LIR) family. CD85J / LILRB1 Contains 4 Ig-like C2-type (immunoglobulin-like) domains. CD85J / LIR-1 is expressed predominantly on B-cells and monocytes. CD85J is receptor for class I MHC antigens and recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles and is also receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. CD85J / LILRB1 interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. CD85J / LILRB1 inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable