SRP6446

CXADR/CAR human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): CAR4/6, HCAR
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 24.9 kDa; observed mol wt 35 kDa by SDS-PAGE (reducing) (Leu 20 is the predicted N-terminal .)
• packaging: pkg of 10 and 50 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P78310
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CXADR(1525)

Description

General description

Coxsackie virus and adenovirus receptor (CXADR) also known as CAR, is a type I transmembrane glycoprotein for group B coxsackie viruses and subgroup C adenoviruses, and belongs to the CTX family of the Ig superfamily. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins such as: fibronectin, agrin, laminin-1 and tenascin-R. Human CXADR protein contains a signal sequence, a extracellular domain (ECD) with a V­ type (D1) and a C2­ type (D2) Ig­like domain, a transmembrane segment and a intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions, and is necessary and sufficient for adenovirus binding. Variants of CXADR are attached to the cell membrane by a GPI- anchor.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable