SRP6377

CD33 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): gp67
• UNSPSC Code: 12352200

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 27.6 kDa; observed mol wt 40-45 kDa by SDS-PAGE (reducing) (Asp 18 is the predicted N-terminus.)
• packaging: pkg of 10 μg
• UniProt accession no.: P20138
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CD33(945)

Description

General description

Myeloid cell surface antigen CD33, (cluster of differentiation 33) also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, belongs to the CD33-related SIGLEC gene family. It is a type 1 transmembrane protein and contains two immunoglobulin-like extracellular domains, a single transmembrane area and two intracellular inhibitory motifs.[1] The CD33 gene is mapped to human chromosome 19q13.[2]

Biochem/physiol Actions

Association of CD33 (cluster of differentiation 33) with sialic acid-containing ligands causes suppression of proinflammatory pathways in monocytes and macrophages.[1] It is mainly involved in anti-inflammatory signaling, cell adhesion and endocytosis functions. CD33 also works as a myeloid differentiation marker.[3] Mutations in the CD33 gene are associated with Alzheimer′s disease susceptibility and acute myeloid leukemia treatment efficacy.[4][5] In microglia, increase in the expression of CD33 decreases amyloid β peptide phagocytosis.[3]

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable