SRP6376

CD273 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): Butyrophilin B7-DC, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 23.4 kDa; observed mol wt 35-48 kDa (DTT-reduced. Protein migrates due to glycosylation. Leu 20 is the predicted N-terminus.)
• packaging: pkg of 10 μg; pkg of 50 μg
• UniProt accession no.: Q9BQ51
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... PDCD1LG2(80380)

Description

General description

Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as butyrophilin B7-DC or CD antigen CD273. It belongs to the immunoglobulin superfamily or BTN/MOG family. The gene encoding CD273 is localized on human chromosome 9p24.1.[1]

Biochem/physiol Actions

The expression of programmed cell death 1 ligand 2 (PD-L2) or CD antigen 273 (CD273) is up-regulated by interferon-γ (IFNG/IFN-γ) stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with colony-stimulating factor-2 (CSF2) and interleukin-4 (IL-4).[1] PD-L2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner.[2] PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.[3]

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable