SRP6246

TPO from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

• Synonym(s): MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: rat
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 18.7 kDa
• packaging: pkg of 10 μg; pkg of 50 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P49745
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: rat ... TPO(81811)

Description

General description

TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from Sodium Citrate, pH 4.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable