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SRP6140

Sigma-Aldrich

hSUMO1-AMC human

≥95% (SDS-PAGE)

Synonym(s):

Small ubiquitin-related modifier 1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

11.142

packaging

pkg of 50 μg

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SUMO1(7341)

General description

hSUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.

Physical form

Lyophilized powder

Preparation Note

Centrifuge the vial prior to opening.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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