SRP6127
BLMH human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym(s):
BH, BLM hydrolase, BMH, Bleomycin hydrolase
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
General description
BLMH (bleomycin hydrolase) is a neutral cysteine protease, belonging to the papain superfamily. It shares structural similarity to 20S proteasome. It is expressed ubiquitously. This gene is localized to human chromosome 17q11.2, and codes for a 455-residue protein.[1]
Biochem/physiol Actions
BLMH (bleomycin hydrolase) deaminates bleomycin, a glycopeptide anticancer agent, by hydrolyzing the amide bond in the β-aminoalanine moiety, subsequently inactivates it. It is responsible for the generation of natural moisturizing factors (NMF). This protein also participates in homocysteine-thiolactone metabolism, antigen presentation, and amyloid-β-peptide clearance.[1][2] BLMH is a candidate for β-secretase, which is responsible for the production of β-amyloid (Aβ) peptide. Aβ aggregation is one of the key events in Alzheimer′s disease (AD) pathogenesis. The G/G genotype of BLMH is linked with sporadic AD.[3]
Physical form
1 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Preparation Note
Centrifuge the vial prior to opening.
Other Notes
MGSSHHHHHH SSGLVPRGSH MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM GALAE
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Dong Won Lee et al.
Scientific reports, 10(1), 21039-21039 (2020-12-05)
The niacin-responsive repressor, NiaR, is transcriptional repressor of certain nicotinamide adenine dinucleotide (NAD) biosynthetic genes in response to an increase in niacin levels. NAD is a vital molecule involved in various cellular redox reactions as an electron donor or electron
Bleomycin hydrolase is regulated biphasically in a differentiation- and cytokine-dependent manner: relevance to atopic dermatitis.
Kamata Y, et al.
The Journal of Biological Chemistry, 286(10), 8204-8212 (2011)
Bo Liu et al.
Scientific reports, 5, 7678-7678 (2015-01-09)
The recombinant Hc proteins of botulinum neurotoxins and tetanus toxin are exclusively produced by intracellular heterologous expression in Pichia pastoris for use in subunit vaccines; the same Hc proteins produced by secreted heterologous expression are hyper-glycosylated and immunologically inert. Here
Confirmation of the association between bleomycin hydrolase genotype and Alzheimer's disease.
Papassotiropoulos A, et al.
Molecular Psychiatry, 5(2), 213-215 (2000)
D R Schwartz et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(8), 4680-4685 (1999-04-14)
The papain superfamily member bleomycin hydrolase (Blmh) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent, is deaminated in vitro by Blmh. We used gene targeting to generate mice
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