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Key Documents

SRP6048

Sigma-Aldrich

HAT (P/CAF) human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Synonym(s):

E1A-associated protein p300, HAT, Histone acetyltransferase p300, p300 HAT

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

assay

≥95% (SDS-PAGE)

form

lyophilized

impurities

Endotoxin, tested

NCBI accession no.

shipped in

wet ice

Storage temp.

−70°C

Gene Information

human ... PCAF(8850)

General description

Recombinant P/CAF is useful in HAT inhibitor screening, kinetic studies and HAT substrate cleavages in vitro or in tissue culture. P/CAF can also be used as positive control in HAT activity assay. The recombinant P/CAF (p300/CBP-associated factor) encoding human sequence from 492 to 658 of accession number Q92831 was produced from E. coli and then purified by several chromatographic steps.

Physical form

Lyophilized powder

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in MilliQ H2O to a concentration of 1 mg/ml. The solution can then be used in bioassay, or store at -20°C for future use.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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