SRP6046

Glucokinase from human pancreas

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(s): Hexokinase, Hexokinase, pancreatic, Pancreatic glucokinase
• CAS Number: 9001-51-8
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human pancreas
• recombinant: expressed in E. coli
• assay: ≥80% (SDS-PAGE)
• form: liquid
• mol wt: 53.1 kDa
• packaging: pkg of 10 μg
• concentration: 1 mg/mL
• impurities: Endotoxin, tested
• NCBI accession no.: P35557
• UniProt accession no.: P35557
• shipped in: dry ice
• Storage temp.: −70°C
• Gene Information: human ... GCK(2645)

Description

General description

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.

Physical form

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable