SRP6040

TIMP-1 human

recombinant, expressed in Sf9 cells, ≥92% (SDS-PAGE)

• Synonym(s): TIMP, TIMP recombinant, TIMP-1 protein, Tissue Inhibitor of matrix metalloproteinases-1
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in Sf9 cells
• assay: ≥92% (SDS-PAGE)
• form: liquid
• mol wt: 28.0 kDa
• packaging: pkg of 5 μg
• manufacturer/tradename: Croda International PLC
• impurities: Endotoxin, tested
• NCBI accession no.: P01033
• shipped in: wet ice
• storage temp.: −70°C
• Gene Information: human ... TIMP1(7076)

Description

General description

TIMP1 (tissue inhibitor of metalloproteinase 1) is a glycoprotein and is a member of TIMP family of endogenous MMP (matrix metalloproteinase) inhibitors. Humans contain four TIMPs. All TIMPs contain an N-terminal of 125 residues, a 65-residue C-terminal, and both these domains contain three disulfide bonds. The N-terminal domain inhibits MMPs by folding into a separate unit.[1] TIMP1 is encoded by the gene mapped to human chromosome Xp11.23.[2]

Biochem/physiol Actions

TIMP1 functions as a poor inhibitor of MT1 (membrane type 1)-MMP, MT3-MMP, MT5-MMP and MMP-19. It inhibits ADAM10 (a disintegrin and metalloproteinase domain-containing protein 10) protein. In human umbilical vein endothelial cells (HUVECs), the down-regulation of TIMP1 and up-regulation of MMP-3 results in aberrant endothelium-dependent vasodilation, EC (endothelial cell) death, and endothelial interruption in a FOXO3 (forkhead box O3)-mediated manner. In patients with CAD (coronary artery disease) and acute coronary syndrome (ACS), the urine levels of this protein are elevated.[1] This protein interacts with Bcl-2 (B cell lymphoma) protein, and induces apoptosis in lung adenocarcinoma cells.[3] The plasma levels of this protein are increased in patients with obesity and obesity-related disorders, such as steatosis, where it participates in pathogenesis of diet-induced hepatic steatosis and glucose intolerance.[4]

Physical form

In 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl?; 1 ?M ZnCl?; 0.05% Brij^® L23; 0,05% NaN?

Legal Information

Brij is a registered trademark of Croda International PLC

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable