SRP6026

UbcH5a human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(s): UbcH
• CAS Number: 1622402-25-8
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: liquid
• mol wt: 17.0 kDa
• packaging: pkg of 10 μg
• concentration: ~1.0 mg/mL
• impurities: Endotoxin, tested
• NCBI accession no.: P51668
• shipped in: dry ice
• Storage temp.: −70°C
• Gene Information: human ... UB2D1(7321)

Description

General description

UbcH5a is highly homologous to the S. cerevisiae Ubc4/5 family of E2 enzymes demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5a, together with UbcH5b and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors. Specifically, UbcH5a stimulates the conjugation of ubiquitin to the tumor suppressor p532. Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 μM depending upon conditions.

Physical form

Supplied at a concentration of 1.0 mg/ml in 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable