SRP5260

SETD8, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): PR-Set7, SET07, SET8
• CAS Number: 1418304-29-6
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~68 kDa
• NCBI accession no.: BC050346
• application(s): genomic analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SETD8(387893)

Description

General description

SETD8 or SET domain containing lysine methyltransferase 8 is a lysine methyltransferase that monomethylates p53 in human cell lines. The monomethylation by SETD8 suppresses p53-mediated transcriptional activation of highly responsive target genes, such as p21 (CDKN1A) and PUMA (BBC3) but it has little influence on weak p53 targets. Depletion of SET8 augmented the proapoptotic and checkpoint activation functions of p53, and SETD8 expression is downregulated during DNA damage. SETD8 is a Wnt signaling mediator and is recruited by LEF1/TCF4 to regulate the transcription of Wnt-activated genes, possibly through H4K20 monomethylation at the target gene promoters.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable