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SRP5231

Sigma-Aldrich

CDC7/DBF4 active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

ASK, CDC7L1, CHIF, DBF4A, HsCDC7, Hsk1, MGC117361, MGC126237, MGC126238, ZDBF1, huCDC7

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About This Item

CAS Number:
649786-18-5
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

7.2-9.8 nmol/min·mg

mol wt

~125 kDa (DBF4)
 ~94 kDa (CDC7)

NCBI accession no.

NM_003503
NM_006716

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CDC7(8317) , DBF4(10926)

General description

CDC7 is a cell division cycle 7 homolog protein that is critical for the G1/S transition and is also essential for initiation of DNA replication as cell division occurs. CDC7 is expressed in many normal tissues, but the overexpression of CDC7 may be associated with neoplastic transformation for some tumors and transformed cell lines. CDC7/DBF4 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 that evolved to integrate several protein kinase.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
I363-1

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Yi-Jun Sheu et al.
Nature, 463(7277), 113-117 (2010-01-08)
Eukaryotic DNA replication uses kinase regulatory pathways to facilitate coordination with other processes during cell division cycles and response to environmental cues. At least two cell cycle-regulated protein kinase systems, the S-phase-specific cyclin-dependent protein kinases (S-CDKs) and the Dbf4-Cdc7 kinase
G F Hess et al.
Gene, 211(1), 133-140 (1998-05-29)
The Cdc7 protein kinase of Saccharomyces cerevisiae is a critical regulator of several aspects of DNA metabolism and cell cycle progression. We describe the isolation of a human gene encoding a Cdc7 homolog. The Cdc7Hs protein sequence is 27% identical

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