SRP4987

Otoraplin (OTOR) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): FDP, Fibrocyte-derived protein, MIAL, Melanoma inhibitory activity-like protein, OTOR, Otoraplin
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: ~12.7 kDa
• packaging: pkg of 20 μg
• impurities: endotoxin, tested
• NCBI accession no.: Q9NRC9
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... OTOR(56914)

Description

General description

TOR proteins is also known as fibrocyte-derived protein (Fdp) and Melanoma inhibitory activity-like (MIAL). Otoraplin is a member of the melanoma-inhibiting activity gene family. Otoraplin is a secreted 16 kDa globular protein that is expressed in the inner ear by periotic mesenchyme and developing and mature fibrocytes. OTOR is highly homologous to MIA/cartilage-derived retinoic acid-sensitive protein (CD-RAP), which is a cartilage-specific protein that is also expressed in malignant melanoma cells. The 111 amino acid mature human otoraplin contains 1 SH3 domain (46 –107 amino acids) and a Tyr at position 50 that is reportedly sulfated. Otoraplin takes part in the initiation of periotic mesenchyme chondrogenesis. Otoraplin is secreted through the Golgi apparatus and plays a role in cartilage development and maintenance. A frequent polymorphism in the translation start codon of OTOR can abolish translation and may be associated with forms of deafness. Recombinant human Otoraplin produced in E. coli is a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12.7 kDa.The OTOR is purified by proprietary chromatographic techniques.

Physical form

The OTOR protein was lyophilized from a concentrated (1 mg/ml) solution containing 20 mM PBS pH-7.4 and 130 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in sterile dH₂O to a concentration of 0.1 -1 mg/mL. This solution can then be diluted into other aqueous buffers.