SRP4964

VEGF121 murine

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)

• Synonym(s): Vegf, Vegf120, Vegf164, Vegf188, Vegfa, Vpf
• MDL number: MFCD01636149
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: rat
• recombinant: expressed in E. coli
• assay: ≥97% (HPLC); ≥97% (SDS-PAGE)
• form: lyophilized
• potency: 1-5 ng/mL
• mol wt: ~28.2 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: NP_033531
• UniProt accession no.: Q00731
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... VEGFA(7422)

Description

General description

Vascular endothelial growth factor 121 (VEGF121) belongs to the platelet-derived growth factor (PDGF)/VEGF family characterized by the presence of eight conserved cysteine residues and a cystine knot structure.[1] VEGF121 is a disulfide-linked homodimer containing 121 amino acids and is the only variant of VEGF that does not interact with heparin.[2]
Recombinant Mouse VEGF-121 is a truncated version of Mouse VEGF-165.

Biochem/physiol Actions

VEGF121/rGel (toxin gelonin) fusion plays a vital role in targeted destruction of tumor vasculature in vivo and it can be used as a potent anti-cancer agent in cancer treatment. In mice, overexpression of VEGF121 enhanced the tumor growth, whereas VEGF165 decreased tumor growth, without affecting cancer cell proliferation in culture.[2]

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute the lyophilized product with sterile water at a concentration of 0.1 – 0.5 mg/mL, which can be further diluted into other aqueous solutions.