SRP4805
Thrombospondin human
recombinant, expressed in baculovirus infected Sf9 cells, ≥98% (SDS-PAGE)
Synonym(s):
THBS, THBS1, TSP, TSP1, Thrombospondin-1
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About This Item
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biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
assay
≥98% (SDS-PAGE)
form
lyophilized powder
mol wt
~180.0 kDa
packaging
pkg of 10 μg
pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TSP1(7057)
General description
The gene TSP1 (thrombospondin 1) is mapped to human chromosome 15q14.[1] It is a matricellular glycoprotein and can be secreted by many cells.[2][3][4] Recombinant human thrombospondin is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. Human thrombospondin is purified by proprietary chromatographic techniques.
Application
In rat C6 astroglioma cells, TSP1 (thrombospondin 1)-human has been used in coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression.[5] It has also been used in the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.[6]
Biochem/physiol Actions
TSP1 (thrombospondin 1) is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair.[7][8] TSP1 expression is highly regulated by different hormones and cytokines and is developmentally controlled. TSP1 stimulates the growth of vascular smooth muscle cells and human foreskin fibroblasts. A combination of IFN-γ (interferon γ) and TNF-α (tumor necrosis factor α) inhibits TSP1 production in these cells.[9] In endothelial cells, it controls adhesion, migration as well as proliferation. It also exhibits antiangiogenic properties and regulates immune processes.[2][3] TSP1 binds to various cell surface receptors, such as integrins and integrin-associated protein CD47.[7] It also plays a crucial role in inflammatory processes and post-inflammatory tissue dynamics.[4] TSP1 has been used as a potential regulator of tumor growth and metastasis.[3] It is upregulated in rheumatoid synovial tissues and might be associated with rheumatoid arthritis.[10] Variants of this gene might be linked with increased risk of autism.[11]
Physical form
Lyophilized from 20 mM sodium phosphate, pH 6.0, 300
mM NaCl and 0.1% BSA.
mM NaCl and 0.1% BSA.
Reconstitution
Reconstitute with water at the concentration 0.1 mg/ml. Avoid freeze-thaw cycles.
Storage Class
13 - Non Combustible Solids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Identification of promoter region methylation patterns of MGMT, CDKN2A, GSTP1, and THBS1 genes in intracranial meningioma patients.
Genetic Testing and Molecular Biomarkers, 16, 335-340 (2012)
Thrombospondins as matricellular modulators of cell function.
P Bornstein
The Journal of clinical investigation, 107(8), 929-934 (2001-04-18)
Common and rare variants of the THBS1 gene associated with the risk for autism.
Psychiatric Genetics, 24, 235-240 (2014)
Thrombospondin-1 suppresses wound healing and granulation tissue formation in the skin of transgenic mice.
Streit M
The Embo Journal, 19, 3272-3282 (2000)
Upregulation of Thrombospondin 1 Expression in Synovial Tissues and Plasma of Rheumatoid Arthritis: Role of Transforming Growth Factor-?1 toward Fibroblast-like Synovial Cells.
The Journal of Rheumatology, 42, 943-943 (2015)
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