SRP4642

VEGF-B human

recombinant, expressed in E. coli

• Synonym(s): VEGFB, Vascular Endothelial Growth Factor-B
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 0.5-5.0 μg/mL
• mol wt: ~19.63 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: P49765
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... VEGFB(7423)

Description

General description

Vascular endothelial growth factor B (VEGF-B), a member of the VEGF family,[1] is a potent growth and angiogenic cytokine. VEGF-B is expressed in all tissues except the liver.[2] It forms cell surface-associated disulfide linked homodimers and can form heterodimers with VEGF-A. There are two known isoforms, formed by alternative splicing, which have been designated VEGF-B167 and VEGF-B186. Both forms have identical amino-terminal sequences encoding a "cysteine knot" like structural motif, but differ in their carboxyl-terminal domains. Both VEGF-B isoforms signal only through the VEGFR1 receptor. The gene encoding this protein is localized on human chromosome 11q13 and contains seven exons.[2][3] Recombinant human VEGF-B is a 38kDa disulfide-linked homodimeric protein consisting of two 167 amino acid polypeptide chains.

Biochem/physiol Actions

Vascular endothelial growth factor B (VEGF-B) promotes DNA synthesis in endothelial cells, regulates angiogenesis and vascular permeability, and inhibits apoptosis in certain smooth muscle cells and neurons.[2] It has a role in coronary vessel growth and cardiac hypertrophy, which might provide protection to heart against ischemic damage and heart failure. VEGF-B is also involved in neuroprotection. The protein might have a role in metabolic functions since it is highly expressed in tissues with highly active energy metabolism.[1]

Physical form

Lyophilized without any additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in 0.1% acetic acid to a concentration of 0.1-1.0 mg/mL.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable