SRP4591

gAcrp30/Adiponectin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

• Synonym(s): Acrp30, Adipocyte-specific protein AdipoQ, Adipolean, Apm-1, adipoQ, adiponectin
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized
• potency: <1-3 μg/mL
• mol wt: ~16.6 kDa
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: NM_004797
• UniProt accession no.: Q15848
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... ADIPOQ(9370)

Description

General description

Acrp30 (adipocyte complement-related protein), or cytokine adiponectin, is a hormone produced and secreted by differentiated adipocytes. It is a 30kDa hydrophilic protein. This protein is composed of an N-terminal collagenous domain and a C-terminal globular trimerization domain. It exists in plasma predominantly as trimeric, hexameric or other high-molecular weight forms. The globular subunit gAcrp30 is however absent in plasma and is biologically active.[1][2] gAcrp30 is the truncated form of full length of Acrp30, and functions as a ligand of AdipoR1.[3]
The Recombinant Human gAcrp30/Adipolean is a 16.6 kDa protein consisting of 145 amino acid residues

Biochem/physiol Actions

In obese mice and humans, adiponectin mRNA expression is reduced, therefore linking this hormone to energy homeostasis. In incubated mouse muscle and cultured cells, gAcrp30 leads to an increase in fatty acid oxidation. This hormone is also linked to anti-atherogenesis, and in apoE (apolipoprotein)-deficient mice, overexpression of gAcrp30 results in significant attenuation of atherosclerosis. Long-term administration of this hormone in mice results in weight loss without reduction in food intake.[1] This hormone is capable of reducing proliferation of MDA-ERα7 cell line. Studies show that ratio of gAcrp30 to leptin and the ERα (estrogen receptor) status of cells are key in determining BRCA (breast cancer) cell growth.[3]

Physical form

Lyophilized from 10 mM Tris, pH 9.2 plus 0.05% Sarcosyl plus 0.75 mM DTT.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 1 mg/mL. This solution can then be diluted into other aqueous buffers.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable