SRP4189

IL-32 alpha human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE)

• Synonym(s): IL-32α, IL-32β, IL-32γ, IL-32δ, NK4TAIFTAIFaTAIFbTAIFc, TAIFd
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized
• mol wt: ~14.9 kDa
• packaging: pkg of 10 μg
• impurities: <1.0 EU/μg endotoxin, tested
• NCBI accession no.: P24001
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL32(9235)

Description

General description

Human IL-32α is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rβ. IL-32α has been shown to induce IL-8, TNF-α, and MIP-2 production from human and mouse macrophage cell lines. It is up-regulated in activated T- and NK-cells, and IFN-g-treated epithelial cells. Human recombinant IL-32α produced in E. coli is a 131 amino acid non-glycosylated polypeptide with a molecular mass of 14.9 kDa.

Biochem/physiol Actions

Interleukin-32 (IL-32) is a proinflammatory cytokine. It stimulates the generation of interleukin-6 and tumor necrosis factor-α. It also has a role in cell apoptosis and immune responses. IL32 has been associated with inflammatory diseases.[1][2][3]

Physical form

Sterile Filtered white lyophilized (freeze-dried) powder.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL.