SRP3232

RELM-beta from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): Cysteine-rich secreted protein FIZZ2, Resistin-like beta
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: 18.0 kDa
• packaging: pkg of 25 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.2 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: Q99P86
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... RETNLB(57263)

Description

General description

Resistin-like molecule-β (RELM-β), also known as found in inflammatory zone-2 (FIZZ-2), is a member of RELM/FIZZ family of cysteine-rich secretory proteins. Recombinant murine RELM β is an 18.0kDa, consisting of two 83 amino acid residue chains. RELM-β contains highly conserved C-terminal domain and the molecular structure is highly homologous to that of adipose-derived cytokine resistin.[1] It is a disulfide-linked homodimeric protein synthesized and secreted by goblet cells in intestine.[2] RELM-β is expressed in the lumen of the bowel and epithelial cells of the intestine.[1][3]
Recombinant Murine RELM beta is an 18.0kDa, consisting of two 83 amino acid residue chains.

Biochem/physiol Actions

Increased expression of resistin-like molecule-β (RELM-β) in asthmatic airways is associated with pulmonary inflammation and airways remodeling in the mouse. It increases deposition of matrix proteins via enhancing fibroblast proliferation and differentiation.[1] RELM-β is implicated in interleukin 13 (IL-13) induced antiparasitic activity. RELM-β plays a vital role in the regulation of colonic epithelial cell barrier function and gastrointestinal innate immunity. Decreased expression of RELM-β leads to colonic inflammation.[4]

Sequence

MQCSFESLVD QRIKEALSRQ EPKTISCTSV TSSGRLASCP AGMVVTGCAC GYGCGSWDIR NGNTCHCQCS VMDWASARCC RMA

Physical form

Lyophilized from 10 mM Acetic Acid.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319
• pcodes: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable