SRP3193

Cardiotrophin-1 from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): CT-1
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: ≤1.0 ng/mL ED₅₀
• mol wt: 21.3 kDa
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: off-white to yellow
• UniProt accession no.: Q60753
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... CTF1(13019)

Description

General description

Cardiotrophin-1 is from interleukin (IL)-6 family of cytokines. It is mainly present in adipose tissues, osteoclasts and cardiomyocytes.[1][2][3] Cardiotrophin-1 works through gp130 (glycoprotein) and the LIFR (LIF receptor).[2]

Biochem/physiol Actions

In adipocytes, cardiotrophin-1 helps in activating signaling pathways associated with metabolism. It controls energy homeostasis, and glucose and lipid metabolism. High levels of cardiotrophin-1 are reported in obesity and metabolic syndrome.[1] Absence of cardiotrophin-1 in mice causes mature-onset obesity, insulin resistance and hypercholesterolemia.[4] In osteoclasts, it enhances osteoblast activity as well as mineralization.[2] It also participates in cardiomyocyte survival, hypertrophy, cardiac fibroblast proliferation and collagen type I synthesis.[5] Cardiotrophin-1 is associated with arterial fibrosis and stiffness.[3]

Sequence

SQREGSLEDH QTDSSISFLP HLEAKIRQTH NLARLLTKYA EQLLEEYVQQ QGEPFGLPGF SPPRLPLAGL SGPAPSHAGL PVSERLRQDA AALSVLPALL DAVRRRQAEL NPRAPRLLRS LEDAARQVRA LGAAVETVLA ALGAAARGPG PEPVTVATLF TANSTAGIFS AKVLGFHVCG LYGEWVSRTE GDLGQLVPGG VA

Physical form

Lyophilized from 0.5mg/ml in 5mM Tris, pH 8.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable