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SRP3179

Sigma-Aldrich

TSG human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):

Twisted Gastrulation Protein

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50 μG
$395.00

$395.00

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50 μG
$395.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

$395.00

Usually ships in 2 weeks.

biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

≤2.0 μg/mL ED50

mol wt

22.2 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

Q9GZX9

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TWSG1(57045)

General description

Twisted gastrulation protein (TSG) is a secreted bone morphogenetic protein (BMP)-binding protein structurally related to the BMP antagonists chordin and noggin.[1] The gene encoding TSG is localized on human chromosome 18p11.22.[2] Recombinant human TSG is a 199 amino acid 22.2kDa protein containing the BMP/TGFb binding portion of the full length TSG protein.

Biochem/physiol Actions

Twisted gastrulation protein (TSG) can inhibit bone morphogenetic protein (BMP) activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment)[2] by binding to the BMP4/chordin complex.

Sequence

MCNKALCASD VSKCLIQELC QCRPGEGNCS CCKECMLCLG ALWDECCDCV GMCNPRNYSD TPPTSKSTVE ELHEPIPSLF RALTEGDTQL NWNIVSFPVA EELSHHENLV SFLETVNQPH HQNVSVPSNN VHAPYSSDKE HMCTVVYFDD CMSIHQCKIS CESMGASKYR WFHNACCECI GPECIDYGSK TVKCMNCMF

Physical form

Lyophilized from 10 mM Sodium Acetate, pH 6.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0706375

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Minimal evidence for a direct involvement of twisted gastrulation homolog 1 (TWSG1) gene in human holoprosencephaly
Kauvar EF
Molecular Genetics and Metabolism (2011)
The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling
Michael O
Nature (2000)
Andrea Wills et al.
Developmental biology, 289(1), 166-178 (2005-12-03)
In the developing vertebrate embryo, proper dorsal-ventral patterning relies on BMP antagonists secreted by the organizer during gastrulation. The BMP antagonist chordin has a complex interaction with BMPs that is governed in part by its interaction with the secreted protein

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