SRP3175
TL-1A human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
TNFSF15, VEGI
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
10.0-100.0 ng/mL
mol wt
22.0 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TNFSF15(9966)
General description
TL-1A belongs to the TNF superfamily of ligands. It is expressed predominantly in endothelial cells, and to a lesser extent in placenta, lung, kidney, skeletal muscle, pancreas, small intestine and colon. TL-1A inhibits endothelial cell proliferation and angiogenesis, and has been shown to induce NFkB activation, caspase activity, and apoptosis in responding cell lines. TL-1A interacts with TNFRSF25/DR3 receptor, but can also bind to a decoy receptor TNFRSF21/DR6. Recombinant human TL-1A is a 22.0 kDa polypeptide of 194 amino acid residues.
Biochem/physiol Actions
TL-1A belongs to the TNF superfamily of ligands. Recombinant human TL-1A is a 22.0 kDa polypeptide of 194 amino acid residues.
Sequence
QLRAQGEASV QFQALKGQEF APSHQQVYAP LRADGDKPRA HLTVVRQTPT QHFKNQFPAL HWEHELGLAF TKNRMNYTNK FLLIPESGDY FIYSQVTFRG MTSECSEIRQ AGRPNKPDSI TVVITKVTDS YPEPTQLLMG TKSVCEVGSN WFQPIYLGAM FSLQEGDKLM VNVSDISLVD YTKEDKTFFG AFLL
Physical form
Lyophilized from 10 mM Tris, pH 8.5 + 75 mM Arginine.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Yoon-Joong Kang et al.
Cytokine, 29(5), 229-235 (2005-03-12)
TL1A (VEGI/TNFSF15) is the ligand for DR3 (TNFRSF12) and is a newly identified member of the tumor necrosis factor superfamily (TNFSF). Previously, DR3 has been shown to have a role in atherogenesis through stimulation of matrix degrading enzymes including matrix
Li-Jin Chew et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 16(7), 742-744 (2002-03-30)
Vascular endothelial cell growth inhibitor (VEGI), a member of the tumor necrosis factor (TNF) family, is an endothelial cell-specific inhibitor of angiogenesis. Overexpression by cancer cells of a secretable VEGI fusion protein resulted in abrogation of xenograft tumor progression, but
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