SRP3038

FGF-16 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Synonym(s): FGFG, Fibroblast Growth Factor-16
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• potency: ≥2x10^6 units/mg
• mol wt: 23.7 kDa
• packaging: pkg of 25 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: O43320
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... FGF16(8823)

Description

General description

Fibroblast growth factor-16 (FGF-16) is a heparin binding growth factor that is a member of the FGF9 subfamily.[1] Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation.[2] Recombinant human FGF-16 is a 23.7 kDa protein consisting of 207 amino acid residues.

Biochem/physiol Actions

Fibroblast growth factor-16 (FGF-16) signals through FGFR 2c and 3c. FGF-16 plays a role in the development of the central nervous system. This protein has a positive effect on mitosis and cell survival. FGF-16 also has roles in tissue repair, embryonic development, morphogenesis and tumor growth.[2]

Sequence

MAEVGGVFAS LDWDLHGFSS SLGNVPLADS PGFLNERLGQ IEGKLQRGSP TDFAHLKGIL RRRQLYCRTG FHLEIFPNGT VHGTRHDHSR FGILEFISLA VGLISIRGVD SGLYLGMNER GELYGSKKLT RECVFREQFE ENWYNTYAST LYKHSDSERQ YYVALNKDGS PREGYRTKRH QKFTHFLPRP VDPSKLPSMS RDLFHYR

Physical form

Lyophilized from 5 mM sodium phosphate, pH 9.0, containing 0.1 M L-arginine and 0.4 mM DTT.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable