SRP3035

Exodus-2 (CCL21) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): 6Ckine, CCL21, SLC
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 10.0-100.0 ng/mL
• mol wt: 12.2 kDa
• packaging: pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: O00585
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CCL21(6366)

Description

General description

Exodus-2 or C-C motif chemokine ligand 21 (CCL21) is a CC chemokine that signals through the C-C chemokine receptor-7 (CCR7). It is expressed in lymph nodes of certain endothelial cells, and in the spleen and appendix.[1][2] CCL21 contains six cysteine residues, including the four conserved cysteines present in CC chemokines. The gene encoding this protein is localized on human chromosome 9p13.[2] Recombinant human Exodus-2 is a 12.2kDa protein containing 111 amino acid residues.

Biochem/physiol Actions

Exodus-2 aids in the migration of lymphocytes and dendritic cells to lymph nodes. This is observed in ulcerative colitis. It also chemoattracts T and B lymphocytes and inhibits hematopoiesis.[1] The protein may have a role in the metastasis of pancreatic cancer stem cells.[3] CCL21 has been associated with lymph node metastasis in gastric carcinoma.[4]

Sequence

SDGGAQDCCL KYSQRKIPAK VVRSYRKQEP SLGCSIPAIL FLPRKRSQAE LCADPKELWV QQLMQHLDKT PSPQKPAQGC RKDRGASKTG KKGKGSKGCR KTERSQTPKG P

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable