SRP2019

RXR, α human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(s): FLJ00280, FLJ16020, FLJ16733, NR2B1
• UNSPSC Code: 12352200
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~53 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 400 μg/mL
• color: clear colorless
• NCBI accession no.: NM_002957
• UniProt accession no.: P19793
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... RXRA(6256)

Description

General description

RXRA (retinoid X receptor α) is a member of the nuclear receptor (NR) superfamily, which is the largest known family of transcription factors. All NR family members are defined by two structural components- a central, highly conserved, DNA-binding domain (DBD) composed of ∼66 residues, a C-terminal structurally conserved, ligand-binding domain (LBD) of ∼250 residues. The N-terminal is highly variable and differs from receptor to receptor and contains a ligand-independent transactivation domain known as activation function 1 (AF-1). There are three types of RXR- RXRα, β, and γ.[1][2]

Biochem/physiol Actions

Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. The family is defined by two domains: (a) the central, highly conserved, DNA-binding domain (DBD) of approx. 66 amino acids, and (b) the C-terminal, structurally conserved, ligand-binding domain (LBD) of approx. 250 amino acids. In addition to binding to DNA and activating transcription in response to 9-cis retinoic acid, RXR forms heterodimers with the receptors for thyroid hormone (TR), retinoic acid (RAR), vitamin D (VDR), prostanoids (PEAR), and numerous orphan receptors. RXR acts as both activator and repressor of transcription. In the absence of hormone, RXR (homo- or heterodimer) interacts with SMRT (silencing mediator for retinoid and thyroid hormone receptors) and N-CoR (nuclear receptor corepressor) and represses transcription through recruitment of histone deacetylases. In the presence of hormone, RXR interacts with a number of activators including the SRC-1 family, CBP/p300, pCAF and the TRAP complex to target chromatin acetylation and activation of transcription.

RXRA (retinoid X receptor α) functions as a heterodimer partner for multiple nuclear receptors (NR), including constitutive androstane receptor (CAR), the pregnane X receptor (PXR), the peroxisome proliferator-activated receptor-α (PPARα) etc. It also functions as a partner for NRs that are involved in drug and lipid metabolism, and in coordination with MAPK/PI3K (mitogen activated protein kinase/phosphoinositide 3-kinase) is involved in suppression of hepatic detoxification during inflammation.[3] rs10776909 polymorphism in this gene is associated with the pathogenesis of chronic glomerulonephritis (ChGN) and is linked with poorer clinical course of ChGN.[4]

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable