The composition of SRP2003 is as follows: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT, and 0.2 mM EDTA buffer.
SRP2003
TBP (TATA box binding protein) human
recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
Synonym(s):
GTF2D, GTF2D1, HDL4, MGC117320, TFIID
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10 μG
$453.00
$453.00
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10 μG
$453.00
About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
$453.00
Usually ships in 2 weeks.
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biological source
human
recombinant
expressed in E. coli
assay
≥85% (SDS-PAGE)
form
frozen liquid
mol wt
~39 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
200 μg/mL
technique(s)
electrophoretic mobility shift assay: suitable
color
clear colorless
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... TBP(6908)
General description
TBP (TATA-box binding protein) was originally recognized as a part of the class II initiation factor TFIID. Its C-terminal region is phylogenetically conserved, and is composed of 180-amino acids, containing a highly basic segment, the basic repeat, flanked by two direct repeats. The N-terminal domain of this protein is not conserved across species, and differs in number of residues.[1] TBP gene is localized to human chromosome 6.[2]
Biochem/physiol Actions
TBP (TATA-box binding protein) is essential for the optimal initiation of transcription of ribosomal, messenger, small nuclear, and transfer RNAs by all three eukaryotic RNA polymerases. TBP proteins binds to the TATA consensus sequence (TATAa/tAa/t) with high affinity, through its C-terminal or core region, and identifies minor groove segments and introduces significant DNA deformation. It is a component of the class II initiation factor TFIID, along with TBP-associated factors (TAFIIs), which is crucial for nucleating the assembly of Pol II pre-initiation complex (PIC). PIC is essential for the transcription initiation by RNA polymerase II (Pol II).[1][3] Amplification of the CAG/CAA trinucleotide repeats in TBP gene results in an autosomal dominant cerebellar ataxia, SCA17 (spinocerebellar ataxia type 17), which is characterized by ataxia, dystonia, parkinsonism, and chorea.[2]
TBP is the core protein of the transcription factor IID complex, the multiprotein DNA-binding factor that coordinates activities necessary for initiation of transcription by RNA polymerase II.
The TATA-binding protein (TBP) is believed to function as an essential factor of the general transcription machinery and to be involved in transcription by all three eukaryotic RNA polymerases (pol I, II, and III). TBP specifically binds to TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor suppressor proteins.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Crystal structure of a human TATA box-binding protein/TATA element complex.
Nikolov DB, et al.
Proceedings of the National Academy of Sciences of the USA, 93(10), 4862-4867 (1996)
The Pathogenic Role of Low Range Repeats in SCA17.
Shin JH, et al.
PLoS ONE, 10(8) (2015)
A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription.
Tora L
Genes & Development, 16(6), 673-675 (2002)
Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.
A Hoffmann et al.
Nucleic acids research, 19(22), 6337-6338 (1991-11-25)
TBP, a universal eukaryotic transcription factor?
N Hernandez
Genes & development, 7(7B), 1291-1308 (1993-07-01)
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Could you please provide the buffer composition for the protein suspension for SRP2003-10UG and SRP2028-10UG? I intend to use them for a labeling experiment.
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